Three measurements were taken with the eye tracker and the retest function engaged (method A), and three measurements were taken without the eye tracker and without the retest function (method B). RNFL thickness was measured in 56 normal and 47 glaucomatous eyes by one operator within one session with a brief rest between measurements. To evaluate the impact of Spectralis self-acting eye-tracking (eye tracker) and retest software on the reproducibility of retinal nerve fiber layer (RNFL) thickness measurements in glaucomatous and healthy control eyes by SD-OCT. SD-OCT and TD-OCT measurements correlated, but values were not interchangeable. Conclusions SD-OCT was easier to obtain than TD-OCT in children. Among eyes with severe glaucoma, 4 of 13 (30%) had unreliable TD-OCT but reliable SD-OCT. SD-OCT measured lower RNFL and higher macular thickness than TD-OCT. Same-day SD-OCT and TD-OCT measures in 53 eyes correlated linearly (RNFL r 2 =0.88 MVr 2 =0.58). Results Included were 83 eyes of 83 children, mean age 11.9±4.2 years. Children with neurologic disorders, refractive error >±5D, pseudophakia and prematurity were excluded. SD-OCT values were compared to TD-OCT(OCT-3, Carl-Zeiss-Meditec, Dublin, CA) values in a subset of subjects who had same day scans using both instruments. The peripapillary retinal nerve fibre layer (RNFL) and macular thickness and volume (MV) were compared amongst four groups: normal eyes, eyes with physiologic cupping (C:D >0.5 and 21) and severe glaucoma (C:D>0.5, IOP>21). Methods Children who had SD-OCT(Spectralis, Heidelberg-Engineering,Germany) were retrospectively and prospectively identified from Duke paediatric glaucoma clinic. The aim of the study was to assess the utility of SD-OCT in paediatric glaucoma and determine its agreement with TD-OCT. Background/aims Spectral-domain (SD)-Optical Coherence Tomography (OCT) can track eye movements, has faster acquisition time and higher resolution than time-domain(TD)-OCT.
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